Description

This is a small package meant to analyze pressure clamp data generated in HEKA patchmaster software using python. Currently, the package includes p50 and single- channel analysis.

Installation

This package can be installed by running the following command in your terminal:

pip install pressureclamp

Examples

Plot raw or preprocessed pressure clamp data. Then fit and analyze the corresponding pressure-response curves.

Mouse Piezo1 p50 analysis. — **Fig 1.** *Left.* Baseline subtracted current traces from mouse Piezo1. *Right.* Corresponding pressure-response relationship fitted with a Boltzmann sigmoid.

Mouse Piezo1 single-channel analysis. — **Fig 2.** *Left.* Single-channel current traces of mouse Piezo1. *Right.* Double gaussian fit of an isolated single channel trace.

Note

To see an example analysis pipeline see the Jupyter Notebook in the examples folder. You can view the notebook online with interactive plots by going to https://nbviewer.org/ and pasting the following url:

https://github.com/neuro-myoung/PressureClampAnalysis/blob/5d5caf1db7a36e06912cb533b474fb1b7ad54898/examples/pressure_clamp_analysis.ipynb

Roadmap

This package was made for training purposes but if you would like to request specific features please email me at neuromyoung@gmail.com.